In preparation of a series about setting up ELISA-style-binding-assays, I programmed a little tool, which helps to predict ELISA curves.
If you have an idea about the KD you can easily determine how many datapoints (samples) at which concentration you need to get a well defined curve. You can also easily compare the effects of different serial dilution regimes and different hill coefficients. Hopefully this helps to better plan your next ELISA style binding assay!
You can find it in the menu under >Tools< ore directly here.