Plate layout & proper controls

This entry is part 1 of 1 in the series ELISA

In scientific experiments in general and in ELISA-style binding assay in particular, proper controls are of utmost importance. Without these an interpretation of the final result is often impossible. In planning a proper plate layout, you ensure that the signals you see, are really caused by your protein interaction and not by non-specific binding (NSB is a general problem in many interaction-assay we will later spend and whole article on.)

To detect non-specific binding, you need at least these controls: read more

ELISA-style-binding assay

This entry is part 2 of 1 in the series ELISA

ELISA-style-binding-assays (here shortly called ELISA) are very robust and relatively simple established assays. They are extremely useful for making single points measurements, and thereby testing a lot of proteins for interactions. With a little bit more effort, it’s also possible to extract proper binding constants.

With a small series of posts, I’d like to help with the most important steps in planning and executing an ELISA assay.

Here the most important pro and cons of an ELISA assay in a table. read more