ELISA-style-binding-assays (here shortly called ELISA) are very robust and relatively simple established assays. They are extremely useful for making single points measurements, and thereby testing a lot of proteins for interactions. With a little bit more effort, it’s also possible to extract proper binding constants.
With a small series of posts, I’d like to help with the most important steps in planning and executing an ELISA assay.
Here the most important pro and cons of an ELISA assay in a table.